Understanding imaging spontaneous and evoked calcium activity of the same identified requires examining multiple perspectives and considerations. Model-based decoupling of evoked and spontaneous neural activity in .... Abstract The pattern of neural activity evoked by a stimulus can be substantially affected by ongoing spontaneous activity. Separating these two types of activity is particularly important for calcium imaging data given the slow temporal dynamics of calcium indicators. Imaging spontaneous and evoked calcium activity of the same identified ....
Download scientific diagram | Imaging spontaneous and evoked calcium activity of the same identified neurons during the awake and anesthetized state. Frontiers | Simultaneous Two-Photon Voltage or Calcium Imaging and .... Similarly, here, we used two-photon voltage or calcium imaging combined with simultaneous multi-channel electrical recordings to record spontaneous and stimulus-evoked activity from the primary somatosensory cortex of anesthetized and awake mice. Protocol for calcium imaging and analysis of hippocampal CA1 activity ....
In this protocol, we provide detailed instructions for using single-photon calcium imaging to record and analyze hippocampal neuronal population activity elicited by non-spatial stimuli in mice. Assessing spontaneous sensory neuron activity using in vivo calcium imaging. Here, we have examined whether in vivo calcium imaging with GCaMP6s can be used as an alternative approach.
We show that spontaneously active calcium transients can be visualised in the fourth lumbar dorsal root ganglion (L4 DRG) via in vivo imaging in a mouse model of pain. Here we present a statistical model that decouples stimulus-driven activity from low dimensional spontaneous activity in this case. Plateau depolarizations in spontaneously active neurons detected by .... Our investigation revealed that the three optical indicators tested, OGB1-AM, GCaMP6f, and ArcLightD, exhibited the strongest responses to electrical signals that combine APs with plateau... It's important to note that, spontaneous Ca2+ events are linked to the development of neuronal ....
Using a fluorescent Ca2+ -sensitive Oregon Green probe, we revealed three different amplitude distributions of spontaneous Ca 2+ events (SCEs) in neurons between 15 and 26 days in vitro (DIV) culture maturation. Electrically-evoked oscillating calcium transients in mono- and co .... Here, we characterize astrocytic oscillating calcium transients (OCa 2+ Ts) in mono- and co-cultures of iPSC astrocytes as well as iPSC SN-astrocyte co-cultures on 48 well plate MEAs. We demonstrate that astrocytes exhibit OCa 2+ Ts in an electrical stimulus amplitude- and duration-dependent manner.
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